Search results for " mammalian"
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
Proteins are produced by gene expression in bacterial or mammalian cell culture. Cell-culture media are composed of essential raw materials that are required for cell growth and expression. Similar ce…
Article
Changing the Culture of Cell Culture: Applying Best Practices and Authentication to Ensure Scientific Reproducibility
Immortalized cancer cell lines isolated from various human and other mammalian tissues have been used for decades across multiple areas of biomedical research (6). Their use is central to most drug-di…
Article
Selecting a Comprehensive Bioburden Reduction Plan
For example, mammalian cell lines or formulation constituents may require routine monitoring for mycoplasma or viruses. Open process operations are more susceptible to contamination than are closed pr…
Article
Use of Multivariate Data Analysis in Bioprocessing
Another group employed unsupervised PCA and PLS to analyze data from inline Fourier Transform-near-infrared (FT-NIR) spectroscopy of a mammalian cell culture process for identification of batch homoge…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Recombinant protein production in the biopharmaceutical industry mostly relies on fed-batch mammalian cell culture (1, 2). Often in fed-batch processes, the feed volume can be more than 1000 L for a 5…
Article
Outsourcing Trends in Biopharmaceutical Manufacturing
The results over the past 10 years tell show that outsourcing is not only increasing across traditional systems including mammalian cell culture and microbial fermentations, but also across newer syst…
Article
Evolution of the Monoclonal Antibody Purification Platform
Monoclonal antibodies expressed in yeast
While mammalian cells (e.g., such as Chinese hamster ovary [CHO]), have been the primary expression system for mAb products; other expression systems such …
Article
Elucidating Biosimilars Characterization
Biological molecules are also manufactured by processes involving living organisms, such as genetically engineered bacteria, yeast, or mammalian cells, and although these processes can be well-defined…
Article
Stirring up the fermentation game
Bioreactors designed for production in mammalian culture fall short of meeting these unique requirements, for example in terms of oxygen transfer capacity and temperature control. The Xcellerex XDR-50…
Article
QbD and PAT in Upstream and Downstream Processing
Vanden Boom (Hospira): Parameters such as temperature, pH, osmolality, and dissolved oxygen have the potential to impact the CQAs of mAbs and other mammalian cell-culture derived products. However, wi…