Search results for " TFF"
Article
Perfusion Culture Using TFF or ATF as Cell Retention Method
Click here to read the article >>
Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility…
Article
Downstream Processing for Cell-Based Therapies
However, this technique, although suitable for the purposes of the study, is not a scalable technique like TFF. Other groups have used TFF and expanded-bed chromatography (EBC) as continuous, integrat…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
We found that:
- Cytiva™ superSEC resin enriched exosomes from impurities compared to legacy Sepharose™ CL-2B resin—a well-established resin for exosome purification—after the initial TFF step.
…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
As Young points out, reusable systems can typically require more than 12 hours from set-up to tear-down, while single-use TFF systems require half that time.
Another area where single-use is gainin…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Process Chromatography: Continuous Optimization
A salt-tolerant resin can be very beneficial to a purification process by decreasing the need for dilution or eliminating TFF steps all together,” she notes.
“Next-generation IEX resins that offer…
Article
Unifying Continuous Biomanufacturing Operations
…angential-flow filtration (TFF), comments Morbidelli, although he says the scale-up for both ATF and TFF remains, to a certain extent, an open issue. Bonham-Carter notes that using ATF for cell reten…
Article
Downstream Processing Continues to Worry CMOs
Indeed, a majority of CMOs are considering using single-use disposable TFF membranes and single use-prepacked columns. It remains to be seen the extent to which these will help. Downstream continues t…
Article
Tools for Continuous Bioprocessing Development
External hollow-fiber systems such as the ATF and TFF couple hollow-fiber filters to a conventional bioreactor. These systems circulate cells between the conventional bioreactor and the hollow fibers …
Article
Bioprocess filter selection guide
Filter selection can be a daunting task, and with so many options available, it’s hard to know what is right for your process. This new bioprocess filtration selection guide can help get you o…