Search results for "MAB purification"
Article
A Closer Look at Affinity Ligands
The high specificity and ease of operation of Protein A ligand affinity chromatography has made it the technology of choice and the “darling” of the mAb purification platform process in industry.
Cu…
Article
A Look at the Affinity Chromatography Landscape
The mild pH elution of these resins allows the traditional mAb purification processes to be applied to low-pH labile mAbs or proteins engineered with Protein A binding domains,” Murray notes.
T…
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Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com
Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…
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Comparing Protein A Resins for Monoclonal Antibody Purification
As a consequence, downstream processing must accept and handle higher titers of mAbs in harvested cell-culture fluid (HCCF), and vendors of mAb purification technologies must develop chromatography re…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Early Platform Description
The MAb purification platform used by Pfizer for early-phase clinical supplies has evolved over the last five years to accommodate increases in titer and associated i…
Article
New Therapies Present Scaling Challenges
The downstream purification process can be scaled up, as the purification process for a gene therapy can use similar hardware as is used in mAb purification.
“In general, purification processe…
Article
Process Chromatography: Continuous Optimization
He adds that they are also commonly used in polishing steps during mAb purification.
Multimodal resins most often separate molecules based on ligand properties, but there are examples of multimoda…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
If used correctly, these new analytical methods can reduce analysis and product development time.
ABSTRACT
Monoclonal antibodies represent a significant portion of sales in the biopharma…
Article
Development of Purification for Challenging Fc-Fusion Proteins
…of commercial mAbs (6-10), which typically consist of two or three chromatographic column steps for mAb purification that allow for the purification of similar molecules with minimal development and …
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
An approach for establishing the CQAs of a mAb product by evaluating impact and uncertainty during risk assessment.
A critical quality attribute (CQA) has been defined as “a physical, chemical, …