Search results for "MAB purification" in Articles / App Notes
Article
Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
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Developing a HIC polishing step for removal of mAb aggregates
This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.
It includes:
- The use of HTPD plate…
Article
Exotoxin Clearance from mAb Samples in a Two-Step Chromatography Process
A heat-stable exotoxin can remain as a contaminant of a drug substance throughout the whole manufacturing process, with the risk of adversely affecting patients’ health. One such toxin is cereulide …
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Connected, Automated Downstream Process for mAb Screening and Evaluation
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A pre-clinical lab is often faced with the need to constantly evaluate possible ways of increasing capacity and throughput. This study was done to show both proof of p…
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HTPD: Optimizing a mAb Chromatography Polishing Step
Development of robust purification processes requires significant time and resources. In addition, steadily increasing demands from regulatory authorities for better understanding and control of man…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Newer classes of biotherapies will require innovations in processing technology.
By Glen R. Bolton, Bernard N. Violand, Richard S. Wright, Shujun Sun, Khurram M. Sunasara, Kathleen Watson, Johna…
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Evolution of the Monoclonal Antibody Purification Platform
Various authors have described cation-exchange chromatography as a capture step in mAb purification (8-11). Though it may not be as efficient as Protein A in terms of selectivity of HCP, it can be use…
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A Closer Look at Affinity Ligands
The high specificity and ease of operation of Protein A ligand affinity chromatography has made it the technology of choice and the “darling” of the mAb purification platform process in industry.
Cu…
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A Look at the Affinity Chromatography Landscape
The mild pH elution of these resins allows the traditional mAb purification processes to be applied to low-pH labile mAbs or proteins engineered with Protein A binding domains,” Murray notes.
T…
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Enhancing Resins Addresses Purification Concerns
Christoph Burgstedt/Stock.Adobe.com
Enhancement of resin technology can improve the ability of chromatography to purify the growing landscape of increasingly complex biomolecu…