Search results for " capture"
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
This week Process Development Forum talks about Protein A with Jonathan Royce, business leader for Cytiva chromatography resins.
mAb purification platforms are very well established, and …
Article
Process Chromatography Selection for Downstream Processing Applications
In the case of Fc-fusion proteins, Protein A can still be utilized as a capture step.
Levison (Pall): [Some commonly used separation technologies include] ion-exchange, mixed-mode, hydrophobic int…
Article
Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Most companies have developed standardized purification processes typically consisting of a cell harvest method followed by a capture chromatographic step and one or polishing steps. The approach used…
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
Article
Chromatography for the Diversified Antibody Pipeline
Jeanne Linke Northrop: How is the development we have seen in the antibody therapeutics area effecting chromatography capture that are used in antibody processing?
Eva Helding: The advantage…
Article
Evaluation of Fibro PrismA for Scalable Single-Use mAb Purification
Bayer worked with Cytiva to evaluate a fiber-based alternative, Fibro PrismA, to determine its potential as a truly single-use mAb capture technology. The team assessed scalability from lab- to proces…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Antibody screening often involves capture of an antibody followed by a single injection of antigen. The kinetics of binding can be estimated directly from a screening experiment and is of major intere…
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
• The increased upstream titers in mAb production seen in recent years may make the Protein A capture step a bottleneck.
• Protein A columns columns are also more prone to bioburden contamination…
Article
Process Chromatography: Continuous Optimization
…ly purified using a platform approach in which protein A chromatography resins are used in the first capture step. Polishing is then achieved using some combination of anion exchange (AEX), cation ex…