Search results for " Hydrophobic interaction chromatogr…" in Articles / App Notes
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
This unwanted modification required an extra process step using hydrophobic interaction chromatography (HIC) to separate these modified molecules from the desired product. E. coli expression often yie…
Article
Modeling the Degradation of mAb Therapeutics
Modeling the Degradation of mAb Therapeutics
Kinetic models can be used to study aggregation and fragmentation to help ensure stability.
By Anurag S. Rathore, Rohit Bansal
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Updating Viral Clearance for New Biologic Modalities
Viral clearance processes and guidance must evolve along with newer biotherapeutic modalities.
By Feliza Mirasol
Ensuring viral clearance (i.e., removal) in downstream purification has becom…
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The Challenge of Disruptive Technologies in Bioprocessing
Thermo Fisher Scientific aims to address this challenge with a series of commercialized hydrophobic interaction chromatography resins with a differentiating range of hydrophobicity, resolution, high c…
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A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
Process Development Forum speaks with Professor Alois Jungbauer, Department of Biotechnology, BOKU, Vienna, about Hydrophobic Interaction Chromatography (HIC).
What is your view of HIC in the bi…
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Analyzing Proteins Using SEC, MALS, and UHPLC
Light scattering analysis combined with more rapid size exclusion chromatography improves protein characterization.
The characterization of proteins involves identification of their complex …
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Addressing the Complex Nature of Downstream Processing with QbD
“Polishing steps such as ion exchange, hydroxyapatite, and hydrophobic interaction chromatography can be more heavily influenced by pH, conductivity, loading capacity, residence time, and temperature,…
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Predicting Progress in Protein Aggregation
Techniques to enable the design and formulation of stable, protein-based therapeutics.
In silico analysis and the evaluation of formulability, aided by new analytical tools such as hydrogen deut…
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Protein Purification Troubleshooting Guide
Practical advice to significantly impact your chromatography workflow
Put this poster on your lab wall to quickly identify and resolve protein purification problems when and where they happen. I…
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Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
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