Search results for "cho" in Articles / App Notes
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Development of Purification for Challenging Fc-Fusion Proteins
…electric point (pI) ranging from four to seven were expressed in recombinant Chinese hamster ovary (CHO) cells and partially purified through ProA capture chromatography. The load for the Fc-fusion P…
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Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
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News: Cytiva Adds ELISA Kit to End-to-End HCP Monitoring Capabilities
Cytiva is pleased to announce the new Amersham HCPQuant CHO kit, a generic for detecting host cell protein impurities in drug samples produced in CHO cell lines.
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Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
Beyond that, Racher says, there is increased incorporation of synthetic biology paradigms into Chinese hamster ovary (CHO)-based expression platforms, moving the industry beyond simply increased titer…
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Managing Residual Impurities During Downstream Processing
It incorporates choice of method; aspects of assay performance to be evaluated to demonstrate method validity, including critical reagent identification, retention, and planning; and provides general …
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Tools for Continuous Bioprocessing Development
Generally, production teams choose the platform that affords them the greatest control of the various process parameters. Without design rules for processes, this inevitably results in choosing platfo…
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Fed-batch supplements to boost cell culture performance
Cultures of Chinese hamster ovary (CHO) cells and the cholesterol-sensitive NS0 murine myeloma cell line were fed with HyClone Cell Boost™ nutrient supplements and HyClone LS1000 cholesterol supplemen…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Three recombinant Chinese hamster ovary (CHO) cell lines, developed in-house to produce three different monoclonal antibodies, were used in this study. Cell lines A and B are dihydrofolate reductase-d…
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Evolution of the Monoclonal Antibody Purification Platform
Anion exchange (AEX) chromatography in a flow-through mode is the method of choice for removal of host-cell impurities. Hydrophobic-interaction chromatography (HIC) in bind and elute mode has been use…