Search results for " buffers"
Article
Efficient and Scalable Buffer Preparation
This article describes how to ensure a scalable process for buffer management from process development to manufacturing with inline conditioning (IC). It also explains how Ä…
Poster
Scaling In-Line Buffer Preparation from Process Development to Manufacturing
Preparing buffers for large-scale chromatography requires significant resources: time, money, and storage space. Inline conditioning (IC) is one possible way to address these challenges. At the same t…
Article
Four Purification Steps with Buffer Dilutions in One Go? No Problem!
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Free up time by automating the protein purification and by reducing manual interactions. Learn how Shin Isogai at Biozentrum, Basel, harnesses automation to puri…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Monitoring and Control of Inline Dilution Processes
…here is not much time invested in looking at alternatives such as automated solutions and outsourced buffers,” Vermunt observes.
An effective evaluation will include investigation of the total …
Article
Process Chromatography Selection for Downstream Processing Applications
The process performance, for example aggregate removal on a cation exchanger, may be significantly impacted by minor changes in the conductivity or pH of the buffers. It is important to consider these…
Article
Technology Innovations Improve Process Chromatography Performance
Biomanufacturers consider several key factors when selecting a best-fit process chromatography technology for their products, including the stability of the biologic, compatibility of the buffers used…
Article
Implementing Inline Conditioning to Advance Process Intensification
With inline conditioning buffers can be prepared inline from buffer concentrates or stock solutions, and directly integrated with the chromatography or filtration step. This eliminates the need for in…
Article
Modeling the Degradation of mAb Therapeutics
In brief, buffers examined included those commonly used for Protein A chromatography, cation exchange chromatography, and anion exchange chromatography.
Samples were kept at three different te…
Article
Improving Process-Scale Chromatography
In-line concentration solutions
A number of technologies have been introduced to help address the challenges associated with the preparation and storage of the large volumes of different buffers r…