Search results for " buffers" in Articles / App Notes
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Monitoring and Control of Inline Dilution Processes
…here is not much time invested in looking at alternatives such as automated solutions and outsourced buffers,” Vermunt observes.
An effective evaluation will include investigation of the total …
Article
Process Chromatography Selection for Downstream Processing Applications
The process performance, for example aggregate removal on a cation exchanger, may be significantly impacted by minor changes in the conductivity or pH of the buffers. It is important to consider these…
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Technology Innovations Improve Process Chromatography Performance
Biomanufacturers consider several key factors when selecting a best-fit process chromatography technology for their products, including the stability of the biologic, compatibility of the buffers used…
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Implementing Inline Conditioning to Advance Process Intensification
With inline conditioning buffers can be prepared inline from buffer concentrates or stock solutions, and directly integrated with the chromatography or filtration step. This eliminates the need for in…
Article
Modeling the Degradation of mAb Therapeutics
In brief, buffers examined included those commonly used for Protein A chromatography, cation exchange chromatography, and anion exchange chromatography.
Samples were kept at three different te…
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Improving Process-Scale Chromatography
In-line concentration solutions
A number of technologies have been introduced to help address the challenges associated with the preparation and storage of the large volumes of different buffers r…
Article
What’s New in Upstream Technologies
Wet, one-point standardization before startup allows for the sensor’s characteristics and functionality to be verified before any solutions or buffers are added to the bioreactor bag. The standardizat…
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Development of Purification for Challenging Fc-Fusion Proteins
Equilibration with five-column volume was performed followed by varying the load-ratio conditions and chase with equilibration buffers. Fractions of the flow-through were collected during the loading…
Article
Making the Move to Continuous Chromatography
The manufacturer just needs the right equipment and expertise; the processes use the same buffers and resins as used in their current batch processes,” he says. “Ultimately, if you want to work faster…