Search results for " plates"
Article
Top Tips for Successful Development of Antibody Chromatography Processes
So for instance, PreDictor™ plates, plates, a 96-well format, where you can screen 96 different conditions in the same experiment. There are also small, miniature columns that can be utilized in the s…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
Three different screening techniques using PreDictor™ plates are compared and discussed. Selected conditions were applied in column step elution experiments and antibody yield, pool volume and reducti…
Article
Modeling and Miniaturization: Key Topics at Successful HTPD Conference
HTPD was mainly limited to 96-well plates filled with chromatography resins used for resin selection and initial screening of running condition. Since then, HTPD in chromatography applications has bee…
Article
Mechanistic Modeling of Preparative Ion-Exchange Chromatography
By Anurag Rathore, Vijesh Kumar
Among the various downstream processing unit operations that are used for purification of biotech products, chromatography is uniquely capable of providing resolu…
Article
Developing Cleaning-in-Place Protocols
Cleaning conditions for chromatography media can be screened in a couple of working days using 96-well microtitre plate formats. A study with a Protein A resin illustrates the approach in this appli…
Article
Design of Experiments for Analytical Method Development and Validation
Design of experiment is a powerful development tool for method characterization and method validation.
Design of experiments (DOE) is a well-proven characterization approach within product…
Article
Developing a HIC polishing step for removal of mAb aggregates
It includes:
- The use of HTPD plates to select the optimal resin as well as running conditions
- The use of a design of experiments (DoE) to optimize the running conditions
- A Monte Carlo simul…
Article
Webinar: Optimizing a HIC step with HTPD
This workflow includes the use of high-throughput process development (HTPD) plates to facilitate the choice of resin and initial conditions for column chromatography, and a design of experiments (DoE…
Article
Using Digital Twins to Model Process Chromatography
Mechanistic models provide process understanding for developing robust manufacturing processes and for scale up and tech transfer.
By Jennifer Markarian
A digital twin is a model of a proces…
Article
Best Practices for Selecting a Top-Quality Cell Line
Irina Shi - Stock.Adobe.com
Leveraging automation and a step-by-step approach are keys to success.
By Cynthia A. Challener
Cell lines determine the performanc…