Search results for "plasmid"
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Top Challenges to Overcome When Developing AAV Processes: Part 1 of 2
…ding which development platform to use and scalability
3:09 – Parameters evaluated for AAV plasmid transfection
4:27 – Three main challenges for downstream AAV process development, incl…
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Cell-free Expression Systems Pose Cell Culture Alternative
In addition, Jewett notes that “the ability to readily store, distribute, and activate freeze-dried cell-free systems by simply adding water or one of a catalog of freeze-dried plasmid DNA templates o…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
The use of computation fluid dynamics tools is also helping with optimization of flow rates and the location for plasmid addition to improve productivity.
Single-use bioreactor simplification
…
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Innovative Chromatography Resins Can Improve Purity and Quality
“For instance, when it comes to the purification of truly large molecules, such as large proteins, viral vectors, or plasmids, these molecules may not be able to enter the pores of the beads, resultin…
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Genetic Vaccine Platforms Demonstrate Their Potential
In the case of DNA-based vaccines—either plasmid/naked DNA- or viral-vector-based—explains Bleck, the cell that takes up the DNA will transcribe the DNA into mRNA, and then the mRNA will be …
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Process Development: 2020 Reflections and 2021 Possibilities
More Popular Articles of 2020
The following are some of the year’s best articles from Bioprocess Development Forum:
Purification of Plasmid DNA
How to Pack Lab-Scale Columns
Gene The…
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Virus-like Particles as Therapeutic Moieties of the Future
VLPs have been shown to encapsulate a variety of cargo including foreign DNA, plasmid DNA, RNA (4), genome editing machinery, siRNA, proteins, and peptides for in-vitroand in-vivo applications. Furthe…
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Technology Innovations Improve Process Chromatography Performance
Convective flow membrane chromatography and monoliths addressed the previous purification challenges with diffusive matrices for large molecules, such as viruses (> 100 nm), plasmids, or even large pr…
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Can Vaccine Development Be Safely Accelerated?
The plasmids are then produced in large quantities using bacteria. The overall development and approval timeline is thereby significantly reduced.
Inovio began animal testing of INO-4800, its C…
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Mapping a Route for Cell and Gene Therapy Process Development
He points in particular to the avoidance of the use of plasmids and the development of packaging/stable cell lines as current key issues.
Reducing manufacturing costs, as well as development of…