Search results for " media" in Articles / App Notes
Article
Unifying Continuous Biomanufacturing Operations
John Bonham-Carter, director of upstream sales at Repligen, says Repligen estimates the median run length for new processes in clinical trials today is approximately four weeks, with approximately fou…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
These differences in glycosylation can have significant effects on the quality of the therapeutic protein produced, as can the choice of cell clone, the basal and feed media used, and the cell-culture…
Article
Eliminating Residual Impurities Starts with a Strategic Plan
They can be derived from the host cell or the product itself, media components, surfactants, virus inactivation agents, inorganic or organic contaminants from permanent and single-use process equipmen…
Article
Optimizing Late-Stage and Commercial Cell-Culture Processes
… of better bioreactor processes, including control strategies for pH, carbon dioxide, composition of media, and feeds.
“The switch from serum-containing to protein-free processes was challeng…
Article
Process Chromatography Selection for Downstream Processing Applications
(2) evaluated several high-capacity CEX resins and showed [some] of these media also provided sufficient capacity and host cell protein clearance to replace Protein A in a three-step chromatography pr…
Article
Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
ActiCHO SM media was incubated at 37 °C for three days, followed by four days of incubation at ambient temperature, for a total of seven days of incubation. The rocking parameters were 20 rpm and 6° a…
Article
Viral Clearance Challenges in Bioprocessing
Upstream viral clearance
Using media with a low level of viral contamination can lead to major problems in biologics manufacturing. Cell-culture media and other raw materials, particularly those de…
Article
A Closer Look at Affinity Ligands
…ls, followed by a series of steps to purify the antibody from all the impurities in the cell culture media. Following the removal of cells and cell debris from the media, the first chromatography pur…
Article
Platform Technologies Improve Protein Expression
In addition, according to Mueller-Tiemann, the simplicity of the culture media required for their growth presents opportunities from a cost-reduction perspective. Use of chemically defined media also …
Article
Real Time Continuous Microbiological Monitoring
Numerous studies have shown that there is a large proportion of microorganisms that are viable but unable to grow on the traditional agar media. Therefore, these microorganisms, known as viable but no…