Search results for " concentration"
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
See Figure 1 for a sample of the interaction properties that may affect binding activity and concentration of a bispecific antibody.
Figure 1: Changes of critical quality attributes, such…
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
The assay was shown to be specific for the detection of glucose in all studied multi-component media without matrix interference regardless of glucose concentration or the vendor.
In the past few …
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
Synthetic or chemically defined media are prepared by adding defined concentration of nutrients (both organic and inorganic) such as vitamins, salts, trace elements, carbohydrates, and cofactors. Esta…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Loadings on PC1 were heavily influenced by growth, osmolality, and amino acid concentrations. The feed system of the platform process was linked to the viable cell concentration. The correlation obser…
Article
Development of Purification for Challenging Fc-Fusion Proteins
1]
Analytical techniques
Protein concentration was measured using a NanoDrop 2000 (Thermo Fisher Scientific). Microplate protein concentration analysis was measured using a S…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
…actions
To estimate the actual association and dissociation rates
To measure the concentration of one of the species.
SPR is a rapidly emerging tool for studying ligand …
Article
Modeling the Degradation of mAb Therapeutics
Environmental factors that can have an impact on the protein include pH, temperature, salt concentration, buffer type, protein concentration, ionic strength, mixing, shear, metal ions, pressure, freez…
Article
Monitoring and Control of Inline Dilution Processes
…n best suited for the specific process needs, considering the number of buffers to be used and their concentrations and flow rates. “It is important to compare the different alternatives and understa…
Article
Establishing Acceptance Criteria for Analytical Methods
Traditional measures of analytical goodness include the following:
• % coefficient of variation (CV) = (repeatability/mean)*100
• % recovery = (measured concentration/standard concentration)*100
…
Article
Improving mAb Manufacturing Productivity by Optimizing Buffer and Media Prep Process Flow
With this approach, the manufacturer can quickly dispense the material to make a buffer of a known concentration. This method also eliminates any on-site powder kitting process steps while reducing ho…