Search results for "cell-culture"
Article
Mapping a Route for Cell and Gene Therapy Process Development
In this context, he identifies multiple important challenges:
- Modification of 2D cell-culture systems into a scalable, three-dimensional (3D) suspension-culturesystems with adequate control s…
Article
Perfusion in the 21st Century
By Bruce Lehr, Delia Lyons
Cell-culture technology for biopharmaceutical manufacturing has steadily advanced over the past three decades. Batch processing, in which all raw materials are introduce…
Article
Achieving Process Balance with Perfusion Bioreactors
“For a perfusion process, you may need to add a vessel volume per day of cell-culture media. For a perfusion process run in a 2000-L bioreactor, 2000 L of media may be needed per day vs. 2000 L per ru…
Article
Process Chromatography: Continuous Optimization
Challener
Cell-culture and fermentation are high-yielding processes that effectively produce complex biologic molecules with desirable bioactivities. Even so, there are many impurities presen…
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Single-use Bioreactors Have Reached the Big Time
Improvements in cell-culture processes have led to higher titers and thus higher cell densities, which have facilitated the adoption of single-use bioreactors through reductions in needed reactor volu…
Article
Evolution of the Monoclonal Antibody Purification Platform
This helps in eliminating preconditioning of load from cell-culture harvest. The product can be eluted at lower pH similar to Protein A. Currently, it offers relatively lower binding capacity than Pro…
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A Look into Biologic Scale-Up Strategies
To maximize cell-culture output, strategy is required that takes into consideration factors such as cell culture media, environmental monitoring, and process controls. Yet, despite decades of process …
Article
Design Considerations for a Commercial Cell and Gene Therapy Facility
Viral-vector production, he notes, has a number of parallels with mAb manufacturing, especially if using a suspension cell-culture process or an adherent format that has the footprint of a bioreactor …
Article
Platform Technologies Improve Protein Expression
“High-quality, defined, animal-free reagents and cell-culture products help biomanufacturers eliminate the risk of contamination that has been associated with animal- and human-serum-derived media,” a…
Article
The New World of Biopharmaceutical Manufacturing
And Pall’s iCELLis bioreactor system is an automated fixed-bed bioreactor, designed to simplify adherent cell-culture processes using single-use technology.
Galliher (Cytiva): In the pas…