Search results for " Protein A"
Article
Cleaning Protein A Resins with 1 M NaOH
If not removed, these impurities can build up on the protein A resin and start to leach into the process material in subsequent cycles. The presence of cell culture nutrients in the harvest feed loade…
Article
Affinity chromatography Handbook: Vol. 2 Tagged Proteins
This handbook is intended for those interested in the expression and purification of recombinant proteins. The use of recombinant proteins has increased greatly in recent years, as has the wealth of…
Article
Handbook: Design of Experiments in Protein Production and Purification
Design of experiments (DoE) is a technique for planning experiments and analyzing the information obtained. The technique allows us to use a minimum number of experiments, in which we systematically v…
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
Article
Protein L affinity resin is ready to move to manufacturing
Although protein L affinity resins have been available for more than a decade, insufficient binding capacities and alkaline stability challenges have limited their use in productive manufactur…
Resource
Protein A Fiber Chromatography from Research to Scale-up and Manufacturing of Monoclonal Antibodies
This makes the pre-packed, small-scale Protein A fiber unit a versatile tool for processes such as clone selection and upstream cell-culture media development. The time savings in downstream processes…
Article
Capacity and Performance of a Next Generation Protein A Chromatography Resin
Click here to read application note: Capacity and performance of MabSelect™ PrismA protein A chromatography resin
For more information, visit MabSelect Protein A resins
Article
High Throughput Protein Purification at a Glance
Within the Human Protein Atlas project, scientists are using ÄKTA systems to purify up to 180 protein samples per week. Frida Henningson Johnson, Annelie Cajander, and Anne-Sophie Svensson sha…
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
• The increased upstream titers in mAb production seen in recent years may make the Protein A capture step a bottleneck.
• Protein A columns columns are also more prone to bioburden contamination…
Article
Protein Purification Questions and Answers from ÄKTA Club Web Chat
Do you have recurring discussions in your lab that you need input on? Or do you need support to get on with your protein purification? Around 40 protein purifiers joined our latest ÄKTA club we…