Search results for " buffer"
Article
What’s New in Upstream Technologies
Wet, one-point standardization before startup allows for the sensor’s characteristics and functionality to be verified before any solutions or buffers are added to the bioreactor bag. The standardizat…
Article
Development of Purification for Challenging Fc-Fusion Proteins
A low pH buffer is routinely used to elute the mAb product, which is followed by a viral inactivation step.
Anion exchange chromatography (AEX) (6-8, 10-11), typically serves as a polishi…
Article
Making the Move to Continuous Chromatography
“The real benefit,” asserts Levison, “is overall greater productivity and improved process economics based on reduced resin requirements and reduced buffer usage.”
As a contract manufacturer th…
Article
Fluid Handling in Biopharma Facilities
BioPharm: Which fluids represent the largest volumes within a facility?
Blomberg (Meissner): Typically within a biopharmaceutical manufacturing process that employs single-use systems, buffers and …
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Samples for iCIEF also must undergo additional sample preparation steps, frequently including a buffer exchange before analysis.
Ion Exchange Chromatography
Ion-exchange chromatography (IEC)…
Article
QbD and PAT in Upstream and Downstream Processing
Control of these critical buffers is managed through batch preparation and release prior to use based on acceptable pH and conductivity ranges. Process capability is sufficient to reproducibly prepare…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
Another area where single-use is gaining ground downstream is in buffer preparation, storage, and dispense. “Single-use mixers, coupled with single-use bioprocess containers (BPC), can increase a DSP …
Poster
Scale-up and process economy calculations of a dAb purification process using ready-to-use products
In addition, process economy of using ready-to-use equipment and ready-made buffers versus using reusable equipment and in-house prepared buffers was compared. A three-step dAb purification process wa…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
The cost of the virus-retaining filter, which can approach that of the protein A resin, can be reduced by using prefilters or optimizing protein concentration, temperature, buffer composition, and sol…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Over time, the buffer replaces the analyte and the dissociation rate of the analyte is monitored. Both the association and dissociation curve can be fitted to one of the chosen models. In addition, th…