Search results for " buffer" in Articles / App Notes
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated manner based on methods created …
Article
Monitoring and Control of Inline Dilution Processes
“The advent of buffer concentrates delivered in single-use (SU) bags combined with automated, compact precision dilution and blending technology allows these tank farms to be repurposed for more produ…
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Process Chromatography Selection for Downstream Processing Applications
Some factors companies may consider when selecting a separation technology include the stability of the biologic, buffer compatibility, and ease of scale-up. In this roundtable discussion, industry ex…
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Technology Innovations Improve Process Chromatography Performance
Biomanufacturers consider several key factors when selecting a best-fit process chromatography technology for their products, including the stability of the biologic, compatibility of the buffers used…
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Implementing Inline Conditioning to Advance Process Intensification
With inline conditioning buffers can be prepared inline from buffer concentrates or stock solutions, and directly integrated with the chromatography or filtration step. This eliminates the need for in…
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Modeling the Degradation of mAb Therapeutics
Environmental factors that can have an impact on the protein include pH, temperature, salt concentration, buffer type, protein concentration, ionic strength, mixing, shear, metal ions, pressure, freez…
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Improving Process-Scale Chromatography
Capacity in a capture step such as Protein A is critical in reducing cost and increasing the ease of use through reductions in column size, buffer usage, and overall footprint of the unit operation,” …
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What’s New in Upstream Technologies
Wet, one-point standardization before startup allows for the sensor’s characteristics and functionality to be verified before any solutions or buffers are added to the bioreactor bag. The standardizat…
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Development of Purification for Challenging Fc-Fusion Proteins
A low pH buffer is routinely used to elute the mAb product, which is followed by a viral inactivation step.
Anion exchange chromatography (AEX) (6-8, 10-11), typically serves as a polishi…
Article
Making the Move to Continuous Chromatography
“The real benefit,” asserts Levison, “is overall greater productivity and improved process economics based on reduced resin requirements and reduced buffer usage.”
As a contract manufacturer th…