Search results for "affinity chromatography"
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…
Article
The Challenge of Disruptive Technologies in Bioprocessing
An example of the latter may be the use of affinity chromatography in the capture step of antibody processes, Jagschies says.
“When we look at the biologics market dynamics for treatment of dis…
Article
Preclinical Evaluation of Product Related Impurities and Variants
Preclinical Evaluation of Product Related Impurities and Variants
The approaches for sample preparation of preclinical evaluation of safety and efficacy are addressed taking into consideration the s…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
Optimization of each phase in a chromatographic cycle has a positive impact on productivity.
By Anna Grönberg
An important area of focus for the biopharma industry is the elucidation and dev…
Article
Challenges of Protein Aggregation During Purification
Careful selection of downstream processing conditions is a must.
Sep 1, 2014
By: Cynthia Challener, PhD
BioPharm International
Volume 27, Issue 9, pp. 32-35
Removal of protein a…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Using this process, the pH of the Protein A affinity chromatography eluate was adjusted upward after pH inactivation and loaded directly onto the anion exchange resin. The anion exchange flow-through …
Article
Top challenges in recombinant protein purification process development
Well, for recombinant proteins, you often don't have an affinity solution, an affinity chromatography solution that can be used, which is very different from mAbs, which do have a great affinity solut…
Article
Scaling Up Novel Therapies
Scaling Up Novel Therapies
Platform processes have improved monoclonal antibody scale-up. Can they do the same for personalized therapies?
By Agnes S…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Additionally, ceramic hydroxyapatite (CHA), immobilized metal affinity chromatography (IMAC), and HIC methods were screened with elution gradients.
Due to the low pI of this Fc-fusion protein, a …
Article
Improvements in Protein A Chromatography
Process Development Forum speaks about Protein A chromatography with Glen Bolton, Scientific Director at Amgen. Bolton recently published a paper “Role of more than 40 years of improvement in Protei…