Search results for " cell" in Posters
Poster
Benchmarking, scalability, and protein production in CHO cell culture
With the large number of CHO clones in use, it is important to have a cell culture medium that supports high viable cell counts and productivity with multiple cell clones. A precise balance of nutri…
Poster
Strategies for optimized cell culture media: Effectively using design space to influence product quality
Here we highlight a statistical design strategy to optimize cell culture media to influence the charge variant profile of a mAb produced in CHO DG44 cells.
Media optimization requires incorp…
Poster
Scale-up of CHO fed-batch cultures in HyClone™ ActiPro™ medium supplemented with Cell Boost™ 7a and 7b
Results from shake flask cultures show high viable cell densities and protein production. In addition, scalability of the culture process was demonstrated in 2 and 50 L bioreactor cultures. Cell densi…
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
This work demonstrates the development of a fed-batch process for a Chinese hamster ovary (CHO) cell line producing a monoclonal antibody (mAb). The results emphasize the importance of an appropriate …
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
To mimic more closely the dynamics of a fed-batch culture, we developed a dynamic feeding strategy responsive to the actual nutrient needs of a mAb-producing recombinant Chinese hamster ovary (CHO) ce…
Poster
Repurposing Fed-Batch Media and Feeds for Highly Productive CHO Cell Perfusion Processes
Here, they show that they can obtain culture media that successfully support perfusion cultures in bioreactors at cell-specific perfusion rates (CSPR) below 25 pL/cell/day. High productivities and fav…
Poster
Development of a chemically defined medium for virus vaccine production in a duck suspension cell line
A project was initiated to improve the production process for viral vaccines through the use of the EB66® cell line derived from duck embryonic stem cells and proprietary to Valneva. The primary goa…
Poster
One-step seed culture expansion from one vial of high-density cell bank to 2000 L production bioreactor
This application note describes how perfusion cell culturing can be used to reduce processing time, simplify operations, and maximize equipment utilization in seed culture expansion processes.
Poster
A Scalable Adenovirus Production Process, from Cell Culture to Purified Bulk
In this study, we have combined technical evaluation of process steps and process economy calculations, from AdV production in cell culture to purified bulk product in up to 10 L scale. Human AdV5, ex…
Poster
Adenovirus production in a single-use stirring tank bioreactor system
Anchorage-dependent cells cultured in roller bottles or cell factories are commonly used in these processes. However, scale-up using these techniques is complicated and limited by the surface availabl…