Search results for " alkaline stability"
Article
Alkaline stability of Fc and VH3 binding in protein A resins
In the development of antibody purification protocols, you need to know your Ab molecule and its possible affinity binding sites for the chromatography resins. In this study, we have looked at…
Article
Evaluating Surface Cleanliness Using a Risk-Based Approach
Evaluating Surface Cleanliness Using a Risk-Based Approach
Rinse sample analysis or visual inspection can be correlated to surface cleanliness to replace surface sampling.
By Elizabeth Rivera…
Article
A Look at the Affinity Chromatography Landscape
…portunity to tailor future Protein A resins for specific needs regarding affinities, pH elution, and alkaline stability.
Significant effort has in fact been directed at designing different lib…
Article
Protein L affinity resin is ready to move to manufacturing
The MabSelect™ VL protein L resin now offers improved alkaline stability compared to Capto™ L resin and a two-fold or more increase in dynamic binding capacity (DBC) in certain applications. After com…
Article
Process Economy Simulations on a Protein A Capture Step
MabSelect PrismA is a next‐generation protein A chromatography resin that offers significantly enhanced alkaline stability and capacity compared with its predecessor products. This application note …
Article
Re-use of Protein A Resin: Fouling and Economics
Mar 01, 2015
By Anurag S. Rathore, Mili Pathak, Guijun Ma, Daniel G. Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography ha…
Article
N-Glycan Analysis of Biotherapeutic Proteins
This article summarizes the approaches, challenges, and future perspectives for the characterization of N-glycans in biopharmaceutical products.
By Aled Jones
Protein glycosylation, the addi…
Article
Enhancing Resins Addresses Purification Concerns
“Additionally, these HIC and AEX resins are known to foul when used as a capture step, and alkaline stability of resins should be considered, such as resins based on polymethacrylate (e.g., Bakerbond …