Search results for "HIC" in Articles / App Notes
Article
A Q&A With Prof. Alois Jungbauer: The Role of HIC in Biotech
What is your view of HIC in the biotech industry today and its role in the future?
HIC is a powerful purification technique that is well established and will remain important in the future. It pro…
Article
New hydrophobic interaction chromatography (HIC) handbook
HIC is often used as a next step to purify proteins in high-salt conditions, for instance after ion exchange chromatography or ammonium sulfate precipitation. The role of this technique in protein pur…
Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
An application example is also presented in which aggregate removal results are compared using Capto™ Butyl ImpRes and Butyl Sepharose High Performance resins.
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Article
Webinar: Optimizing a HIC step with HTPD
Webinar date: Wed, Mar 8, 2023 11:00 AM EST (5:00 PM CET)
This webinar discusses a step-by-step workflow for optimizing a hydrophobic interaction chromatography (HIC) step.
Thi…
Article
Optimization of a HIC step for recombinant protein purification
Follow this step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
…
Article
Developing a HIC polishing step for removal of mAb aggregates
This article describes the development of a flow-through (FT) polishing step using hydrophobic interaction chromatography (HIC) to remove mAb aggregates.
It includes:
- The use of HTPD plate…
Article
Development of Purification for Challenging Fc-Fusion Proteins
Monoclonal antibody purification processes exist in different well-established platforms with extensive process performance histories for production of commercial mAbs (6-10), which typically consist …
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Most company pipelines saw a dramatic increase in the number of therapeutic mAb candidates, which in turn resulted in a desire to shorten development timelines. Due to the large doses required, antibo…
Article
Fundamentals of hydrophobic interaction chromatography
…), bispecific mAbs and antibody-drug conjugates (ADC)
* mRNA
* plasmid
New to HIC? Browse this HIC resource library, containing our recently updated handbook, application notes and m…
Article
Process Chromatography: Continuous Optimization
Drug manufacturers are always seeking ways to improve the efficiency and productivity of chromatographic separations. Suppliers of chromatography resins are developing new resin chemistries and bindin…