Search results for "concentration"
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Because the feed dilutes the culture and reduces product concentration, the use of lower feed volumes can improve overall process productivity as well.
One approach of decreasing feed vol…
Article
Automated Concentration and Diafiltration of Multiple siRNA Samples
Once the duplex is confirmed to have minimal excess of either single strand, the siRNA product requires diafiltration and concentration using ultrafiltration methods. These methods remove residual sal…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
Protein recovery was ≥ 99%, after 5‑times concentration and 5‑fold diafiltration. The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the…
Article
Efficient concentration of a low-titer bovine IgG with high recovery in low volume
The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.
Article
Sensitive and Reproducible SPR-Based Concentration and Ligand-Binding Analyses
This article focuses on concentration assays associated with late-stage development and biotherapeutic drug chemical manufacturing and control.
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Article
Biacore Concentration and Ligand-Binding Analyses
In this white
paper, the use of Biacore for active concentration measurements, target binding, and Fc receptor (FcR) analysis is reviewed along with the use of these assays for assessment of drug po…
Poster
Optimization of concentration and diafiltration of a bovine γ-globulin solution
This application note describes the development of a cross flow filtration process for concentration of a bovine
γ-globulin solution.
Article
Concentration and Diafiltration of Cell-Derived, Live Influenza Virus Using 750 C Hollow Fiber Filter Cartridge
Cross flow filtration (CFF) is a technique extensively used in vaccine production and particularly in influenza vaccine manufacturing. In contrast to normal flow (dead-end) filtration (NFF), the fee…
Article
Sensitive and Reproducible SPR-Based Concentration and Ligand-Binding Analyses
This article focuses on concentration assays used for drug product characterization and quality control.
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Article
Connected Polishing and Concentration Under One Automation Method
Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was cr…