Search results for " fed-batch"
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Managing Biomanufacturing Capacity Expectations
For example, concentrated fed-batch (CFB) cell culture has been shown in early experiments to yield products of similar quality compared with those made through traditional fed-batch culture (6). Not …
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Platform Approach Speeds Process Development
New CHO DG44 and CHO K1 host cell lines evaluated at BI and currently implemented into the development platform are showing potential for up to 11 g/L of final product concentrations for platform fed-…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Recombinant protein production in the biopharmaceutical industry mostly relies on fed-batch mammalian cell culture (1, 2). Often in fed-batch processes, the feed volume can be more than 1000 L for a 5…
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Generating a Fully Processed Antibody
The subsequently tagged antibody-producing cell lines were required to consistently produce fully converted antibody at titers greater than 1 gram/L in a traditional fed-batch manufacturing process.
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Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
The selected Cell Boost supplements were thereafter applied in fed-batch cultures to fine-tune their relative ratios. The selected fed-batch culture conditions supported mAb titers of approximately 4 …
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Continuous Manufacturing: A Changing Processing Paradigm
…ge portfolio of short-run manufacturing products, and “scaling process development is easier using fed-batch technologies” (1). Langer also writes that, based on data from his yearly report, “there h…
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Achieving Process Balance with Perfusion Bioreactors
As with batch and fed-batch processes, single-use bioreactors offer numerous other advantages over stainless steel. “Users can eliminate most of the cleaning and validation steps that are associated w…
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Trends in Single-use Bioreactors
Another example of how single-use systems can facilitate continuous processing is the ease with which a single-use bioreactor that has been operated in a fed-batch manufacturing process can be readily…
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Digitalization: The Route to Biopharma 4.0
Glycosylation Flux Analysis Reveals Dynamic Changes of Intracellular Glycosylation Flux Distribution in Chinese Hamster Ovary Fed-Batch Cultures. Metab. Eng. 2017, 43, 9–20. DOI: 10.1016/j.ymben.2017.…
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Biomanufacturing: Demand for Continuous Bioprocessing Increasing
Negative assessments from within the industry of continuous perfusion fed-batch processing overall may reflect a lack of direct exposure or experience with continuous technology.
In BioPlan’s a…