Search results for " bioreactors"
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Being Thorough When Transferring Technology
For example, when single-use bioreactors were a nascent technology to the industry, there were reports that some bags melted on the larger bioreactors. Someone didn’t anticipate the heating and coolin…
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Downstream Processing for Cell-Based Therapies
Given the wide variety of CGTs, an array of culture devices (stirred-tank bioreactors, hollow-fiber/packed-bed reactors, gas-permeable membrane reactors, etc.) are used. Therefore, the harvesting prot…
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Perfusion in the 21st Century
Perfusion was first introduced in the 1980s to enable the production of sensitive proteins that degraded when left in bioreactors under batch and fed-batch conditions. Around the same time, continuous…
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Generating a Fully Processed Antibody
Process scale-up to bioreactors
One of the single tagged cell lines was expanded for bioreactor production to confirm that the cell lines could be scaled with similar results. Duplicate 2-L biorea…
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Analytical Considerations for Successful Upstream Process Development with Spivey and Lane
…ge quantities of cells in a very short period of time and then using that to either inoculate larger bioreactors or run in a continuous manner. That's where PAT comes in handy.
Raman is the pri…
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New Therapies Present Scaling Challenges
Bioreactor size can be increased (scale up), or the number of bioreactors used can be increased (scale out). In addition, mammalian cell culture technology has advanced such that producing multi-kilog…
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Industry Adoption of Single-Use Systems Remains Low
“It is far more common today that biopharma manufacturers are building small economical facilities with several 2000-L single-use bioreactors--20 years ago, the companies were building a facility with…
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Flexible Facilities for Viral Vector Manufacturing
Makowiecki explains that vector production using adherent cells in fixed-bed bioreactors is limited in scale primarily by the size of the fixed-bed bioreactors, and scale-out is used to increase produ…
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Making the Move to Continuous Chromatography
If a manufacturer can reduce its process to a 2000-L bioreactor, or multiple 2000-L bioreactors, facilities become much easier to build with shorter construction times and reduced cost. In this case, …
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Hybrid Bioprocessing: A Q&A with Andrew Sinclair
With the improvements and the rise of SU equipment bioreactors, containers, and physical transfer, a lot of the complexity is removed and even with some reusable equipment, we can still achieve the fa…