Search results for " capture" in Articles / App Notes
Article
Improving Process-Scale Chromatography
The development of new resin chemistries including enhancements to Protein A for monoclonal antibody (mAb) capture, increasing use of automation, implementation of single-use technologies, and explora…
Article
A Look at the Affinity Chromatography Landscape
Resin suppliers have in recent years been challenged to increase the productivity of the capture chromatography process to enable more efficient purification of increasingly high titers. They have als…
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Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
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Enhancing Resins Addresses Purification Concerns
Affinity chromatography resins such as oligo(dT) can be used as a capture step, but these resins are not able to separate single-stranded RNA (ssRNA) from double-stranded RNA (dsRNA), and they have a …
Article
The Need for Advanced Process Modeling for New Therapeutic Biologics
For example, the bioprocess model would have to capture relevant information consistently as well as thoroughly to allow both researchers and IT developers understand the process requirements. The mod…
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Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
The typical platform process is generally comprised of an initial capture step with Protein A-chromatography, a first polishing step with cation-exchange media, and a final polishing step using anion-…
Article
Innovative Chromatography Resins Can Improve Purity and Quality
A generic capture step for mAbs and Fc fusion proteins has been robustly established by classical Protein A affinity resins, says Oliver Schub, director of Business Development at Navigo Proteins, a s…
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Development of Purification for Challenging Fc-Fusion Proteins
… were expressed in recombinant Chinese hamster ovary (CHO) cells and partially purified through ProA capture chromatography. The load for the Fc-fusion Protein A contained HMWs of up to 5% and LMWs a…
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Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
This week Process Development Forum talks about Protein A with Jonathan Royce, business leader for Cytiva chromatography resins.
mAb purification platforms are very well established, and …
Article
Process Chromatography Selection for Downstream Processing Applications
In the case of Fc-fusion proteins, Protein A can still be utilized as a capture step.
Levison (Pall): [Some commonly used separation technologies include] ion-exchange, mixed-mode, hydrophobic int…