Search results for " capture" in Articles / App Notes
Article
Re-use of Protein A Resin: Fouling and Economics
Bracewell
BioPharm International
Volume 3, Issue 28
In the past two decades, Protein A affinity chromatography has remained the de facto capture step for purification of monoclonal antibod…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Most companies have developed standardized purification processes typically consisting of a cell harvest method followed by a capture chromatographic step and one or polishing steps. The approach used…
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants o…
Article
Chromatography for the Diversified Antibody Pipeline
Jeanne Linke Northrop: How is the development we have seen in the antibody therapeutics area effecting chromatography capture that are used in antibody processing?
Eva Helding: The advantage…
Article
Evaluation of Fibro PrismA for Scalable Single-Use mAb Purification
Bayer worked with Cytiva to evaluate a fiber-based alternative, Fibro PrismA, to determine its potential as a truly single-use mAb capture technology. The team assessed scalability from lab- to proces…
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Antibody screening often involves capture of an antibody followed by a single injection of antigen. The kinetics of binding can be estimated directly from a screening experiment and is of major intere…
Article
Process Chromatography: Continuous Optimization
…ly purified using a platform approach in which protein A chromatography resins are used in the first capture step. Polishing is then achieved using some combination of anion exchange (AEX), cation ex…
Article
Unifying Continuous Biomanufacturing Operations
They added that this so-called “culture and capture” approach is expected to “reduce heterogeneity in other product characteristics such as glycation or oxidation with respect to classical batch opera…
Article
Improvements in Protein A Chromatography
Alternative technologies like CEX capture or precipitation often provide lower purity and require more mAb-specific development.
What future directions would you like to see in the development o…
Article
Host-Cell Protein Measurement and Control
In Figure 1, capture antibodies enrich the HCPs from sample and immobilize them to a 96 (or greater)-well plate. Then, detection antibodies, conjugated directly with an enzyme or through a biotin-avid…