Search results for " yield"
Article
Innovative Chromatography Resins Can Improve Purity and Quality
“The problem is that, with every DSP step, product yields decrease, while bioprocessing time and equipment occupancy goes up, along with DSP manufacturing risks,” Schub says.
He also emphasizes th…
Article
Gene Therapies Push Viral Vector Production
“This makes it a popular starting point for gene therapy, although many serotypes continue to struggle with manufacturing challenges, such as low production yields and therefore high cost of goods,” T…
Article
Combining Purification Techniques in a Multistep Approach
…tistep approach, your protein purification will be successful in terms of either high target protein yield or purity.
In this blog post, learn how to select and combine purification techniques to…
Article
The Challenge of Disruptive Technologies in Bioprocessing
Purification can typically include four to five chromatography steps, which results in a product yield that decreases with each step that is added. Because product yield drives the cost of goods (COG)…
Article
Process Chromatography: Continuous Optimization
“Using tangential flow filtration (TFF) between steps reduces product yield and lengthens process times. A salt-tolerant resin can be very beneficial to a purification process by decreasing the need f…
Article
Improving Process-Scale Chromatography
In principle, according to Ljungqvist, it is possible to purify mAbs using any chromatographic technique, but it is difficult to achieve a similar purity, robustness, and as high a yield as generally …
Article
Re-use of Protein A Resin: Fouling and Economics
It has been observed by several researchers that the performance attributes of chromatographic stationary phases such as step yield, host-cell-impurity clearance capability and DNA-clearance capabilit…
Article
Evaluating Design Margin, Edge of Failure and Process
Design space generation is encouraged in new product development.
Sep 1, 2014
By: Thomas A. Little, PhD
BioPharm International
Volume 27, Issue 9, pp. 46-49
A product’s or proc…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Initially, several range-finding experiments were carried out to determine the optimal parameters for each unit operation and to maximize the yield and purity for that particular antibody. We used the…
Article
Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
These target molecules need to be purified to reach required purity and yield.
Selecting an affinity resin for the diverse range of antibodies can be overwhelming. But don’t worry, in this selecti…