Search results for "filtration"
Article
The Development and Application of a Monoclonal Antibody Purification Platform
After harvest, the cells were removed from the cell culture broth by depth filtration. In some cases, the broth was concentrated before the first chromatography step because of facility fit issues. Pr…
Article
Automated Concentration and Diafiltration of Multiple siRNA Samples
Manual crossflow filtration (CFF) steps of the process lacked process robustness and were a source of variation between production runs. The project objective was to streamline the process to improve …
Article
Design Considerations for a Commercial Cell and Gene Therapy Facility
For mAb bioprocessing and viral-vector manufacturing, the equipment needed is similar, such as single-use bioreactors and standard harvest and downstream systems for clarification, tangential flow fil…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
“Clarification via depth filtration or tangential flow membrane filtration (TFF) is just one example.”
As this trend continues, the concept of an end-to-end single-use manufacturing facility has mo…
Article
Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
Article
Connected Polishing and Concentration Under One Automation Method
Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was cr…
Article
Being Thorough When Transferring Technology
…p from lab scale to commercial scale in biologics?
Moscariello and Young (CMC Biologics): Depth filtration and centrifugation are not always scalable for manufacturing operations. However, having…
Article
Efficient concentration of a low-titer bovine IgG with high recovery in low volume
The use of ReadyCircuit™ single-use assemblies allowed for aseptic connectivity of the filtration device. Filtration was performed using ReadyToProcess™ hollow fiber filter cartridges. The described …
Article
Continuous Processing for the Production of Biopharmaceuticals
…er separation technologies such as continuous chromatography, refolding, precipitation, cell lysis, filtration, and aqueous two-phase extraction (3). Table I presents a summary of recent efforts in t…
Article
Instructional Videos for Bioprocess Equipment
Cytiva offers free on-demand instructional videos that will help you with operating bioreactors, chromatography and filtration systems, including flow kit installation on single-use systems. Modules a…