Search results for " polishing"
Article
Top Process Development Trends for 2021 and a Look into 2022
…elopment platform to use and scalability—and a downstream perspective—such as cell lysis and the polishing step for full capsid enrichment. Nonetheless, it was evident in a roundtable discussion of i…
Article
Using Digital Twins to Model Process Chromatography
BioPharm International spoke with Whitelock about how digital twins are used in biopharmaceutical process development, scale up, tech transfer, and manufacturing, particularly chromatographic polishin…
Article
Technology Innovations Improve Process Chromatography Performance
One area of bottlenecking in continuous mAb polishing chromatography is the cation exchange step, which is traditionally operated in bind and elute mode to provide clearance of product-related aggrega…
Article
The Challenge of Disruptive Technologies in Bioprocessing
In addition, there is a need for new resin chemistries for polishing end product because the bioprocessing of complex therapeutic molecules such as ADCs, antibody fragments, and bispecifics are result…
Article
Tools and Solutions for Separation of Charged mAb Variants
A biosimilar is an almost identical version of an originator product. To attain regulatory approval, a comparable quality to the reference product in terms of efficacy, purity, and safety should be …
Poster
Rapid Process Development of a CIEX Step for a Biosimilar
When establishing purification processes for a biosimilar molecule it is important to develop steps that are scalable, selective, and cost-effective.
A cation exchange (CIEX) chromatographic ste…
Article
Continuous Processing for the Production of Biopharmaceuticals
In another publication, researchers extended the aforementioned work by further integrating the capture step with continuous intermediate and polishing steps (5). In that experiment, a continuous low …
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The cation exchange polishing step was removed and the anion exchange chromatography step was changed from a resin-based operation to one involving membrane adsorbers. This increased the loading capac…
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Most companies have developed standardized purification processes typically consisting of a cell harvest method followed by a capture chromatographic step and one or polishing steps. The approach used…
Article
Fundamentals of hydrophobic interaction chromatography
Hydrophobic interaction chromatography (HIC) is a versatile technique that can be used for capture, intermediate purification, or polishing steps.
This technique separates proteins accor…