Search results for " polishing"
Article
Optimization of a two-step purification method for bispecific antibodies
Bispecific antibodies (bsAbs) have gained increasing interest over recent years because they can bind two antigens, offering unique modes of action compared to monoclonal antibodies. New and i…
Article
mRNA: The messenger - LNP formulation
Join us for part 5 of mRNA: the messenger, where we discuss key considerations for the formulation of lipid nanoparticles (LNPs) for mRNA drug delivery, including mixing, encapsulation, polishing and …
Article
Capture of Bispecific Antibodies and Removal of Product-Related Impurities
One potential solution is to initiate polishing at the capture step using differences in avidity. In this article, Cytiva presents data on applications using MabSelect™ VL protein L resin with novel …
Article
Top Challenges to Overcome When Developing AAV Processes: Part 1 of 2
… 4:27 – Three main challenges for downstream AAV process development, including cell lysis and the polishing step for full capsid enrichment
7:28 – Challenges associated with full and empty ca…
Poster
Comparison of Three Different Screening Techniques for Predicting Step Elution Conditions for a Cation Exchanger
Screening of chromatographic conditions can be done in several ways, such as 96-well plates, mini-columns, and tips. It is important that the screening can give an indication of the optimal conditio…
Article
Next Generation Bioprocesses: Mapping Roads to the Future
In downstream processing, it can be intensified capture, in-line virus inactivation, flow through polishing, in-line virus filtration, or single-pass filtration.
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Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
In this study, the hydrophobicities of newer-generation Capto™ HIC resins were compared to Sepharose™ High Performance and Sepharose Fast Flow HIC resins. An application example is also present…
Article
Addressing the Complex Nature of Downstream Processing with QbD
Quality by design brings both challenges and benefits to the development of downstream processes.
By Susan Haigney
natali_mis/Stock.Adobe.com
Regulators have been en…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
Because chromatography resins make up a large portion of the costs in downstream bioprocessing, switching a polishing step from resin chromatography to a single-use membrane column can significantly r…
Article
Integrated Semi-Continuous Process for mAb Production
In brief, the mAb process consists of perfusion cell culture, continuous capture chromatography, viral inactivation (VI), post VI filtration, batch polishing chromatography, and a final pH adjustment …