Search results for " medium"
Article
Tools for Continuous Bioprocessing Development
It requires long-term cultures, and places greater stress on sterile interfaces for the continuous in-flow and out-flow of medium and cells.
For continuous perfusion processes to be efficient, the …
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
The design and coding of such large experiments is demanding and the preparation of large numbers of feeds and/or medium variants requires innovative approaches. The large number of samples produced c…
Poster
Purification platform challenges and strategies for handling of "tricky" MAbs
In the work presented here, capture using high-capacity protein A medium (resin) was followed by polishing using a novel cation exchange chromatography medium.
Article
Use of Multivariate Data Analysis in Bioprocessing
Figure 3 illustrates an example of use of near-infrared spectroscopy/multivariate data analysis (NIR-MVDA) for screening of lots of basal medium powders based on their impact on process performance an…
Article
Quality by design for biotechnology products—part 1
Examples of process-related impurities include host cell DNA, host cell protein, adventitious agents, residual medium supplements such as protein hydrolysates, and components such as nucleases or resi…
Article
Cell-free Expression Systems Pose Cell Culture Alternative
In the prolonged process of biologic production, the dying cells will inevitably release cytosolic content to the culture medium, essentially “contaminating” the batch and introducing heterogeneity. T…
Article
Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
Article
Remote Monitoring and Big Data Advance Upstream Automation
Connected, integrated bioprocessing enterprises with greater data analytics capabilities are coming.
By Cynthia A. Challener
As upstream processing drives toward higher efficiencies in meeti…
Article
Mapping a Route for Cell and Gene Therapy Process Development
Moreover, the product design at the molecular level, the producer cell line, and the production medium can all be limiting factors for harvest titer. Most importantly, appropriate analytical methods a…
Article
Upstream PD Time-Saver: Optimize Early
Identify the right combination of culture medium, feed supplements, and feeding strategy early in upstream process development to conserve precious time and resources.