Search results for " medium"
Article
Real-Time Characterization of Biotherapeutics and Comparability of Biosimilars
Bulk response caused by the medium in which the analyte is dissolved is the major drawback but can be easily avoided with proper reference measurements (7, 8).
Analyte concentrations are measur…
Article
A Look at the Affinity Chromatography Landscape
A new affinity purification procedure using cell surface heparan sulfate proteoglycan and the non-ionic gradient medium iodixanol has also resulted in improved viral infectivity, efficient purificatio…
Article
Scalable Viral Vector Manufacturing
Also, the cell culture medium and any supplements need to be of clinical grade quality. From a regulatory perspective, it is recommended to avoid any raw materials that are of animal origin such as se…
Article
Understanding Validation and Technical Transfer, Part 2
…m various growing regions or should it be single-sourced? What level of roast should be used: light, medium, dark? How will the roasting be controlled? How will the beans be ground, and to what degre…
Article
Understanding Validation and Technical Transfer, Part 3
In any case, there is an incubation period where the cells grow in a medium that changes depending on the process. The incubation period is also variable depending on the process. Then the cells are c…
Article
Platform Technologies Improve Protein Expression
… for protein expression ranging from the choice of cell line, expression plasmid design, cultivation medium, growth conditions, equipment, scalability, stability, and matching purification capability…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Although Protein A resins introduced to the market in the last 10 years withstand low to medium concentrations of sodium hydroxide for cleaning, like 0.1 M sodium hydroxide and in some cases 0.5 M, th…
Article
Sources of Bioburden You Might Have Missed
What better place for a bacterial contamination to grow than in a well-aerated, nutrient-loaded cell culture medium? Downstream operations also have their share of risk. The open handling introduces m…
Article
Downstream Processing for Cell-Based Therapies
A biological safety cabinet usually is needed for the subsequent resuspension step, which involves discarding spent medium and resuspending in a suitable buffer. This process may be repeated multiple …
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
ActiPro cell culture production medium is intended to be used in combination with Cell Boost 7a and 7b supplements to enhance recombinant protein production in fed-batch or perfusion processes. This…