Search results for " medium" in Articles / App Notes
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…d the initial mAb capture step was carried out with an alkali-stabilized, Protein A-derived affinity medium using a standard protocol. An HTPD approach was then taken for the subsequent purification …
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
A common challenge upon cell transfer is the lag phase, during which the cells are adjusting to the new culture medium and little or no increase in cell number is observed. In the 50 L cultivations, t…
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Commercial Production of Gene Therapies Using Suspension or Adherent Cell Lines
By comparison to STRs, FBRs are “segregated” systems where the cells, immobilized in the bed, are decoupled from the liquid medium and regulations, which allows for parameters to be optimized with les…
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Determining Criticality-Process Parameters and Quality Attributes Part I: Criticality as a Continuum
For Stage 1, however, often a qualitative risk assessment evaluating low, medium, and high risk is sufficient to distinguish relative differences in risk.
Continuum of CQAs
Prior to the develo…
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The use of Xcellerex™ mixing system as slurry tank when packing chromatography columns
The capability of the mixing system to keep the chromatography medium (resin) in suspension and the possibility to integrate the system in column packing were studied. The results show that XDM Quad …
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Ensuring Viral Safety of Viral Vaccines and Vectors
These methods primarily target the cell-culture medium before its transfer to the bioreactor as well as raw materials of animal or human origin. Options include high temperature/short time (HTST), C s…
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Evaluating E&L Studies for Single-Use Systems
The risk ratings of each factor will be weighted as in the BPOG example and summed to obtain the final risk score that is then categorized as low, medium, or high. If the risk is classified as low, it…
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Perfusion in the 21st Century
With perfusion technology, fresh medium is continuously introduced while spent medium, which generally contains the API and waste products, is removed. Cells are retained in the bioreactor by a device…
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Maximum Output Starts with Optimized Upstream Processing
On a positive note, parallelization of experiments using automated systems for high-producer clone selection, along with medium optimization and the availability of standard kits, are having an overwh…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
…ctor being manufactured, the type of cells used, and whether the virus is harvested from the culture medium, the cells, or both. The single-use systems most used, according to Chor Sing Tan, strategy…