Search results for " medium" in Articles / App Notes
Article
Gene Therapies Push Viral Vector Production
In addition, the growth factors need to be continuously and manually added/replenished in the medium. This cumbersome process leads to an increase in the chances of errors.
• Cell expansion …
Article
Best Practices for Selecting a Top-Quality Cell Line
The plasmid ratio and medium are also important, Cai observes. “A balanced plasmid ratio usually has dramatic impact on some formats of bispecific antibody cell line development to minimize impurities…
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Optimization of Fed‑Batch Culture Conditions for a mAb‑Producing CHO Cell Line
In the first step, optimal feed combinations were screened by spiking the basal medium with different Cell Boost combination on day 0 of batch cultures. The selected Cell Boost supplements were therea…
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Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Generating a Fully Processed Antibody
Twenty-four clones were selected for further evaluation, eight with high FGE gene copy, eight with medium, and eight with low. The 24 clones were grown in culture for five weeks, and FGE level was ana…
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Tools for Continuous Bioprocessing Development
It requires long-term cultures, and places greater stress on sterile interfaces for the continuous in-flow and out-flow of medium and cells.
For continuous perfusion processes to be efficient, the …
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
The design and coding of such large experiments is demanding and the preparation of large numbers of feeds and/or medium variants requires innovative approaches. The large number of samples produced c…
Article
Use of Multivariate Data Analysis in Bioprocessing
Figure 3 illustrates an example of use of near-infrared spectroscopy/multivariate data analysis (NIR-MVDA) for screening of lots of basal medium powders based on their impact on process performance an…
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Quality by design for biotechnology products—part 1
Examples of process-related impurities include host cell DNA, host cell protein, adventitious agents, residual medium supplements such as protein hydrolysates, and components such as nucleases or resi…
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Cell-free Expression Systems Pose Cell Culture Alternative
In the prolonged process of biologic production, the dying cells will inevitably release cytosolic content to the culture medium, essentially “contaminating” the batch and introducing heterogeneity. T…