Search results for " hydrophobic interaction"
Article
Process Chromatography Selection for Downstream Processing Applications
For these molecules, chromatographic techniques such as ion-exchange, hydrophobic interaction chromatography, and multimodal resins are common. Affinity resins like Capto L (antigen-binding fragments …
Article
A Closer Look at Affinity Ligands
Affinity ligands play an exciting role in the ever-growing sphere of downstream processing. In speaking with BioPharm International®, Phillip Elliott, PhD, associate director of Process Development,…
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Chromatography for the Diversified Antibody Pipeline
You may need mixed mode interactions where both iron exchange and pure hydrophobic interactions occur. We have multimodal resin which is good at aggregate removal which targets product related impurit…
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Tackling Analytical Method Development for ADCs
Feb 01, 2015
By Cynthia Challener, PhD
BioPharm International
Volume 28, Issue 2
The targeted therapy possible with antibody-drug conjugates (ADCs) makes them an attractive class of …
Article
Modeling the Degradation of mAb Therapeutics
This is likely due to salt ionization, which weakens protein structure and decreases stability because of hydrophobic interactions and weakening of electrostatic repulsions as the salt concentration i…
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Predicting Progress in Protein Aggregation
Techniques to enable the design and formulation of stable, protein-based therapeutics.
In silico analysis and the evaluation of formulability, aided by new analytical tools such as hydrogen deut…
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Top Tips for Successful Development of Antibody Chromatography Processes
But if you still have challenging aggregates, you can look at the cation exchange step and change that to a hydrophobic interaction step. That would be Capto Phenyl (high sub) resin, for instance. Tha…
Article
Improving Process-Scale Chromatography
These resins contain simple multimodal ligands with functional groups that interact with the target protein in multiples, including ionic interactions, hydrophobic interactions, hydrogen bonding, etc.…
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A Look at the Affinity Chromatography Landscape
Cytiva has increased the window of operation for the separation of open-circular versus sc pDNA by improving an existing, flexible ligand based on thiophilic and hydrophobic interactions. The new liga…
Article
Impurity Testing of Biologic Drug Products
Process-related variants can be identified, quantified, and differentiated from process-related impurities of cellular origin via techniques such as SEC–HPLC, hydrophobic interaction chromatography (H…