Search results for " anion exchange" in Articles / App Notes
Article
Alternative solutions to separate AAV full and empty capsids using anion exchange
Here we present high-performance anion exchange (AEX) chromatography separation for several AAV serotypes using step-elution mode. Optimized protocols and conditions for both resin and membrane format…
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Evolution of the Monoclonal Antibody Purification Platform
While both the antibody and impurities bind to the anion exchange resin, the impurities are much more tightly bound than in flow-through mode resulting in significantly higher impurity clearance. Load…
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Top Tips for Successful Development of Antibody Chromatography Processes
So, a cation exchange step and an anion exchange step. If we look at this process, the typical step that removes aggregates is the cation exchange step. So let's leave that for now and look at the ani…
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The Development and Application of a Monoclonal Antibody Purification Platform
After cation exchange, diafiltration was performed to prepare the product for anion exchange chromatography in flow through mode, in which impurities such as DNA and some HCPs were captured on the res…
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Development of Purification for Challenging Fc-Fusion Proteins
Development of Purification for Challenging Fc-Fusion Proteins
This study outlines methods for an alternative protein-polishing process for challenging proteins.
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Article
Process Chromatography: Continuous Optimization
Polishing is then achieved using some combination of anion exchange (AEX), cation exchange (CEX), mixed mode, and HIC resins.
“Protein-A based affinity chromatography is very popular and more wide…
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Technology Innovations Improve Process Chromatography Performance
“From a continuous processing perspective, a flow-through cation exchange step is more easily integrated with other product flow-through purification steps, such as anion exchange polishing or virus f…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Several types of chromatographic resins, including cation exchange, anion exchange, HIC, CHA, and IMAC, were evaluated to remove the less active molecules along with other product and process-related …
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New Selection Guide: Ion Exchange Chromatography Columns and Resins
Use these decision trees to find the most appropriate resin for your needs.
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Article
Modeling the Degradation of mAb Therapeutics
In brief, buffers examined included those commonly used for Protein A chromatography, cation exchange chromatography, and anion exchange chromatography.
Samples were kept at three different te…