Search results for " capture"

Poster Development of capture and intermediate chromatography steps for insulin purification
The results indicated that the media of choice for the capture step was Capto™ MMC, a multi-modal cation-exchange resin. Elution conditions were further optimized in packed columns using the Design of…

Article Capture of human single-chain Fv (scFv) fusion protein on Capto L affinity medium
The industry standard for purifying monoclonal antibodies is a platform approach using affinity chromatography with protein A as the capture step. The high purification factor and generic conditions a…

Article High-throughput screening and process development for capture of recombinant pro-insulin from E. coli
PreDictor 96-well prefilled filter plates, Assist software and Tricorn™ 5/50 columns packed with 1 mL media (resins) were used to develop optimized conditions for the capture step. High-throughput scr…

Poster Purification of antibody fragments - a new route for capture
The introduction of Capto™ L, with its protein L ligand, provides a possibility for an industrial platform purification approach for capture of this class of biomolecules.

Article Case Study: Virus Reduction of an Affinity Capture Step
In this study, the virus reduction of an affinity capture step was evaluated in a scale-down column format. The study was performed by a third-party viral testing laboratory in collaboration with a bi…

Article Multidimensional Scale-Up of a Monoclonal Antibody Capture Step, Using ÄKTA™ pilot 600
In this work, a multidimensional scale-up (change of both column diameter and bed height) of a mAb capture step, using the ÄKTA pilot 600 chromatography system, is demonstrated. Column volume per…

Poster High-throughput screening of HIC media in PreDictor™ plates for capturing a recombinant protein from E.coli
…IC media and shows how salt type and salt concentration conditions could be tailored to successfully capture the target compound. In this study the model compound is Green Fluorescent Protein (rGFP) …

Article High-throughput screening of HIC media in PreDictor™ plates for capturing recombinant Green Fluorescent Protein from E. coli
Hydrophobic interaction chromatography (HIC) is a powerful purification technique where the type and density of the ligand, pH and salt of binding conditions, temperature and the nature of the targe…

Article Process Economy Simulations on a Protein A Capture Step
MabSelect PrismA is a next‐generation protein A chromatography resin that offers significantly enhanced alkaline stability and capacity compared with its predecessor products. This application note …

Poster Evaluation of different cation multimodal ligand structures for capture of pro-insulin
The variation of ligand structures that can be attached to a base matrix is large. In this investigation 15 different cation multimodal ligands containing different hydrophobic and anionic groups we…

Next Page