Search results for "TFF"
Article
Perfusion Culture Using TFF or ATF as Cell Retention Method
The work demonstrates perfusion culture of an antibody producing Chinese hamster ovary (CHO) cell line, using either tangential flow filtration (TFF) or alternating tangential flow filtration (ATF) as…
Article
Downstream Processing for Cell-Based Therapies
Tangential flow filtration (TFF) offers an alternative that can also be scaled, automated, fully contained and, generally, involves a lower capital investment and disposables costs, which may make it …
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
Article
Single-Use for Downstream Chromatography: Benefit or Hindrance?
“Clarification via depth filtration or tangential flow membrane filtration (TFF) is just one example.”
As this trend continues, the concept of an end-to-end single-use manufacturing facility has mo…
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the added benefits of the single‑use technology and consistency through automation.
…
Article
Process Chromatography: Continuous Optimization
“Using tangential flow filtration (TFF) between steps reduces product yield and lengthens process times. A salt-tolerant resin can be very beneficial to a purification process by decreasing the need f…
Article
Unifying Continuous Biomanufacturing Operations
In terms of cell retention devices, alternating tangential filtration (ATF) allowed a significant technological step ahead compared with tangential-flow filtration (TFF), comments Morbidelli, although…
Article
Downstream Processing Continues to Worry CMOs
A majority of CMOs are considering:
• Use of high capacity resins
• In-line buffer dilution systems
• Single-use disposable tangential flow filtration (TFF) membranes
• Single use-prep…
Article
Tools for Continuous Bioprocessing Development
For example, perfusion process development experiments (21) using a 4-L working-volume Wave bag and alternating tangential flow (ATF) or tangential flow filtration (TFF) cell-retention filter, consume…
Article
Bioprocess filter selection guide
Filter selection can be a daunting task, and with so many options available, it’s hard to know what is right for your process. This new bioprocess filtration selection guide can help get you o…