Search results for " AAV"
Article
How to develop a scalable rAAV process
In this webinar you will learn:
• Common pitfalls for rAAV processing and ways to overcome the challenges
• How the full and empty AAV capsid separation can be significantly improved with one …
Article
Top Process Development Trends for 2021 and a Look into 2022
They are further categorized into gene and cell therapies that use vectors such as AAVs, adenoviruses, and lentiviruses for delivery; oligo-based therapeutics such as anti-sense DNA, siRNA, mRNA, spli…
Article
Flexible Facilities for Viral Vector Manufacturing
Whereas for in-vivo gene therapies, using mostly AAV, the scale varies depending on the patient population, disease type, and route of injection, from a few 10 L up to 2000 L. For example, for Duchene…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Chromatography has been shown to remove PCV (33), and ion exchange and affinity chromatography have been shown to purify vectors such as AAVs. However, because achieving robust clearance is challengin…
Article
rAAV Production Using HEK293 Cell Line and Transfection Medium
Improving the performance of a scalable AAV process in Xcellerex™ XDR-10 and XDR-200 bioreactor using HyClone™ peak expression medium.
LEARN MORE >>
Article
Optimizing capture and polishing steps in an rAAV purification process
In this article, we show how we developed an efficient purification process for recombinant AAV (rAAV). The chromatography purification described includes affinity capture to maxi…
Article
The Challenge of Disruptive Technologies in Bioprocessing
Thermo Fisher Scientific has commercialized three POROS CaptureSelect AAV affinity resins to establish industrialized platforms for the purification of adeno-associated virus (AAV). The move is meant …
Article
Q&As with Industry Leaders
…ment & Strategy, Cytiva
Insights and Strategies for Successful Development of Scalable AAV Processes: Part 2 of 2
Anki Magnusson, Staff Research Engineer, Protein and Viral Production, …
Article
What is process development?
What is process development?
Biopharma process development comprises the activities that help you create a series of steps to produce a biomolecule – a monoclonal antibody (mAb), recomb…
Article
From cells to purified capsids: How to develop a scalable rAAV process
Adeno-associated virus (AAV) is the most common vector for gene therapy, yet there’s much room to improve production, purification, and analytics. In this webinar we present the latest upd…