Search results for " fed-batch" in Articles / App Notes
Article
Fed-batch supplements to boost cell culture performance
Cell growth and MAb production in the fed-batch cultures were compared with batch cultures. The results demonstrate increased viable cell yields and productivity in the fed-batch cultures.
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Perfusion N-1 – A Way to Improve the Fed-Batch Production Process
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In this article, we show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fol…
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Intensifying your fed-batch process using cryobags
We will show that high-density cryobags can be used to seed a bioreactor at a 500-fold higher seed density, reducing the total process time by up to 9 days.
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Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
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Perfusion in the 21st Century
Batch processing, in which all raw materials are introduced into the bioreactor at the beginning of the cell-culture process, have largely been replaced with fed-batch systems, in which additional nut…
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Unifying Continuous Biomanufacturing Operations
Lawrence Yu, PhD, deputy director in the Office of Pharmaceutical Quality at the Center for Drug Evaluation and Research (CDER), said in a 2016 FDA blog post that processes that take a month with fed-…
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Managing Biomanufacturing Capacity Expectations
For example, concentrated fed-batch (CFB) cell culture has been shown in early experiments to yield products of similar quality compared with those made through traditional fed-batch culture (6). Not …
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Platform Approach Speeds Process Development
New CHO DG44 and CHO K1 host cell lines evaluated at BI and currently implemented into the development platform are showing potential for up to 11 g/L of final product concentrations for platform fed-…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Recombinant protein production in the biopharmaceutical industry mostly relies on fed-batch mammalian cell culture (1, 2). Often in fed-batch processes, the feed volume can be more than 1000 L for a 5…
Article
Generating a Fully Processed Antibody
The subsequently tagged antibody-producing cell lines were required to consistently produce fully converted antibody at titers greater than 1 gram/L in a traditional fed-batch manufacturing process.
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