Search results for " TFF"

Article Perfusion Culture Using TFF or ATF as Cell Retention Method
Perfusion processes have many advantages such as being favorable for product quality and supporting efficient facility utilization by offering the possibility of using smaller bioreactors. Compared …

Article Upcoming Technologies to Facilitate More Efficient Biologics Manufacturing
Typically, TFF cassettes are installed in stainless steel housings that require post-use cleaning and validation of cleaning. The installation and preconditioning steps before process use are time con…

Article Downstream Processing for Cell-Based Therapies
However, this technique, although suitable for the purposes of the study, is not a scalable technique like TFF. Other groups have used TFF and expanded-bed chromatography (EBC) as continuous, integrat…

Article Purification Strategies to Process 5 g/L Titers of Monoclonal Antibodies
As an alternative, TFF can be used to diafilter the pool, achieving the desired pH and lower conductivity, but this involves adding a new unit operation to the process. In addition, this increases the…

Article Single-Use for Downstream Chromatography: Benefit or Hindrance?
As Young points out, reusable systems can typically require more than 12 hours from set-up to tear-down, while single-use TFF systems require half that time. Another area where single-use is gainin…

Article Robust and Reliable Filtration
Automate your tangential flow filtration (TFF) process for consistent performance, significant time savings, and extensive data recording. Discover GE’s single-use automated filtration solution, …

Article Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
This work describes the capabilities of ÄKTA readyflux when used in ultrafiltration/diafiltration (UF/DF) applications. The process was conducted in an automated  manner based on methods created …

Article Process Chromatography: Continuous Optimization
A salt-tolerant resin can be very beneficial to a purification process by decreasing the need for dilution or eliminating TFF steps all together,” she notes. “Next-generation IEX resins that offer…

Article Unifying Continuous Biomanufacturing Operations
…angential-flow filtration (TFF), comments Morbidelli, although he says the scale-up for both ATF and TFF remains, to a certain extent, an open issue. Bonham-Carter notes that using ATF for cell reten…

Article Downstream Processing Continues to Worry CMOs
Indeed, a majority of CMOs are considering using single-use disposable TFF membranes and single use-prepacked columns. It remains to be seen the extent to which these will help. Downstream continues t…

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